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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
06/03/2006 |
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Data da última atualização: |
03/08/2017 |
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Autoria: |
GERMANO, M. G.; MENNA, P.; MOSTASSO, F. L.; HUNGRIA, M. |
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Título: |
RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
International Journal Systematic and Evolutionary Microbiology, Reading, v.56, n. 1, p.217-229, Jan. 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
Genetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N2-fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76T; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts other than soybean. New clusters were also observed for the IGS region. The largest number of differences was detected in the analysis of the 23S rRNA gene, and 16 groups and isolated strains were joined at a very low level of similarity (16 %). In a combined analysis with the three ribosomal regions, the majority of strains isolated from soybean clustered with a similarity of 54 % with type and reference strains of B. japonicum, while most strains isolated from Brazilian indigenous legume species grouped with B. elkanii at a level of similarity of 46 %. All strains were clustered at a very low level of similarity (27 %), and at least two new clusters were clearly defined. These new clusters might be related to intraspecific differences or to novel subspecies, or even to novel species; indeed, strains from one of these clusters show higher 16S rRNA gene sequence similarity to members of the genus Burkholderia. The results obtained in this study emphasize the high level of diversity of symbiotic diazotrophic bacteria in the tropics that still remains to be determined. MenosGenetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N2-fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76T; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts... Mostrar Tudo |
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Thesagro: |
Fixação de Nitrogênio. |
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Categoria do assunto: |
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Marc: |
LEADER 03149naa a2200169 a 4500
001 1468978
005 2017-08-03
008 2006 bl --- 0-- u #d
100 1 $aGERMANO, M. G.
245 $aRFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species.
260 $c2006
520 $aGenetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N2-fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76T; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts other than soybean. New clusters were also observed for the IGS region. The largest number of differences was detected in the analysis of the 23S rRNA gene, and 16 groups and isolated strains were joined at a very low level of similarity (16 %). In a combined analysis with the three ribosomal regions, the majority of strains isolated from soybean clustered with a similarity of 54 % with type and reference strains of B. japonicum, while most strains isolated from Brazilian indigenous legume species grouped with B. elkanii at a level of similarity of 46 %. All strains were clustered at a very low level of similarity (27 %), and at least two new clusters were clearly defined. These new clusters might be related to intraspecific differences or to novel subspecies, or even to novel species; indeed, strains from one of these clusters show higher 16S rRNA gene sequence similarity to members of the genus Burkholderia. The results obtained in this study emphasize the high level of diversity of symbiotic diazotrophic bacteria in the tropics that still remains to be determined.
650 $aFixação de Nitrogênio
700 1 $aMENNA, P.
700 1 $aMOSTASSO, F. L.
700 1 $aHUNGRIA, M.
773 $tInternational Journal Systematic and Evolutionary Microbiology, Reading$gv.56, n. 1, p.217-229, Jan. 2006.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 15 | |
| 1. |  | CAMPO, R. J.; MOSTASSO, F. L.; HUNGRIA, M. Eficiência agronômica do inoculante Biagro em pré-inoculação da soja. In: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIA DE INOCULANTES MICROBIANOS DE INTERESSE AGRÍCOLA (RELARE), 13., 2006, Londrina. Anais... Londrina: Embrapa Soja, 2007. p. 23. (Embrapa Soja. Documentos, 290). Organizado por Rubens José Campo, Mariângela Hungria.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Soja. |
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| 2. | | MOSTASSO, F. L.; COLOZZI-FILHO, A.; HUNGRIA, M. Efeito de exsudatos de soja, feijão, milho e trigo sobre a germinação e crescimento micelial de Gigaspora gigantea "in vitro". In: SIMPÓSIO BRASILEIRO SOBRE MICROBIOLOGIA DO SOLO, 3.; REUNIÃO DE LABORATÓRIOS PARA RECOMENDAÇÃO DE ESTIRPES DE RHIZOBIUM E BRADYRHIZOBIUM, 6., 1994, Londrina. Resumos... Londrina: IAPAR, 1994. Não paginado. Trabalho n. 106.| Biblioteca(s): Embrapa Soja. |
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| 4. | | CAMPO, R. J.; MORETTI, E. M.; MOSTASSO, F. L.; MIURA, L. M.; HUNGRIA, M. Recuperação de células de Bradyrhizobium em semente de soja inoculada, armazenada e tratada com fungicidas e micronutrientes. In: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIA DE INOCULANTES MICROBIANOS DE INTERESSE AGRÍCOLA (RELARE), 13., 2006, Londrina. Anais... Londrina: Embrapa Soja, 2007. p. 51-52. (Embrapa Soja. Documentos, 290). Organizado por Rubens José Campo, Mariângela Hungria.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Soja. |
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| 5. | | CAMPO, R. J.; MORETTI, E. M.; MOSTASSO, F. L.; MIURA, L. M.; HUNGRIA, M. Recuperação de células de Bradyrhizobium em semente de soja tratada inoculada e armazenada. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82).| Biblioteca(s): Embrapa Soja. |
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| 7. | | HUNGRIA, M.; ARAUJO, R. S.; MOSTASSO, F. L.; CAMPO, R. J. Efeito dos fungicidas na sobrevivência de Bradyrhizobium e na nodução e fixação biológica do nitrogênio com a cultura da soja: incompatibilidade com a aplicação na semente e dose de inoculante para a aplicação no sulco. In: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIA DE INOCULANTES MICROBIOLÓGICOS DE INTERESSE AGRÍCOLA, 15., 2010, Curitiba. Anais... Brasília, DF: Embrapa, 2012. p. 53-54.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 8. | | CAMPO, R. J.; ARAÚJO, R. S.; MOSTASSO, F. L.; HUNGRIA, M. Efeito da população de células na nodulação e rendimento da soja. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 30., 2008, Rio Verde. Resumos... Londrina: Embrapa Soja, 2008. p. 302-304. (Embrapa Soja. Documentos, 304). Organizado por Odilon Ferreira Saraiva, César de Castro, Regina Maria Villas Bôas de Campos Leite, Fábio Alvares de Oliveira.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 10. | | CAMPO, R. J.; ARAUJO, R. S.; MOSTASSO, F. L.; HUNGRIA, M. Efeito da população de células na nodulação da soja. In: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIA DE INOCULANTES MICROBIOLÓGICOS DE INTERESSE AGRÍCOLA, 14., 2008, Bonito. Anais... Dourados: Embrapa Agropecuária Oeste, 2010. p. 43. RELARE. Organizado por Fábio M. Mercante, Oscar F. de Lima Filho, Suelma P. da S. Bonatto.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 12. | | CHUEIRE, L. M. O.; BANGEL, E. V.; MOSTASSO, F. L.; CAMPO, R, J.; PEDROSA, F. O.; HUNGRIA, M. Classificação taxonômica das estirpes de rizóbio recomendadas para as culturas da soja e do feijoeiro braseada no sequenciamento do gene 16S rRNA. Revista Brasileira de Ciência do Solo, Viçosa, v. 27, n. 5. p. 833-840, set./out. 2003.| Biblioteca(s): Embrapa Soja. |
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| 13. | | TORRES, A. R.; ARAÚJO, W. L.; CURSINO, L.; HUNGRIA, M.; PLOTEGHER, F.; MOSTASSO, F. L.; AZEVEDO, J. L. Diversity of endophytic enterobacteria associated with different host plants. The Journal of Microbiology, Korea, v. 46, n. 4, p. 373-379, Ago. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 14. | | HUNGRIA, M.; CHUEIRE, L. M. de O.; CAMPO, R. J.; FERREIRA, M. C.; BANGEL, E.; MOSTASSO, F. L.; PEDROSA, F. de O.; SOUSA, L. J.; CONCEIÇÃO, R. B. Caracterização genética, fisiológica e bioquímica de estirpes de Bradyrhizobium para a cultura da soja de solos da região sul e do cerrado e com maior eficiência de fixação do nitrogênio e capacidade competitiva (04.1994.322-02). In: EMBRAPA SOJA. Resultados de pesquisa da Embrapa Soja, 1999. Londrina, 2000. p. 220-225. (Embrapa Soja. Documentos, 142).| Biblioteca(s): Embrapa Soja. |
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| 15. | | CHUEIRE, L. M. de O.; BANGEL, E.; FERREIRA, M. C.; GRANGE, L.; CAMPO, R. J.; MOSTASSO, F. L.; ANDRADE, D. de S.; PEDROSA, F. O.; HUNGRIA, M. Classificacao taxonomica, baseada na caracterizacao molecular, das estirpes de rizobio recomendadas para as culturas da soja e do feijoeiro. Londrina: Embrapa Soja, 2000. 32 p. (Embrapa Soja. Boletim de Pesquisa, 3).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 15 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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